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KMID : 0380020050200060464
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Korean Journal of Biotechnology and Bioengineering
2005 Volume.20 No. 6 p.464 ~ p.469
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pH-dependent Metabolic Flux Shift in Novel Hydrogen-Producing Bacterium Enterobacter sp. SNU-1453
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Shin Jong-Hwan
Yoon Jong-Hyun
Ahn Eun-kyung
Shim Sang-Jun
Kim Mi-Sun
Park Tai-Hyun
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Abstract
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For the biological production of hydrogen, a new fermentative hydrogen-producing bacterium, Enterobacter sp. SNU-1453, was isolated from a domestic landfill. During the culture of this bacterium, pH significantly decreased with the accumulation of various organic acids, and consequently this inhibited the production of hydrogen. It was found that the metabolic flux in this bacterium depended on the pH and affected the hydrogen production. A butanediol pathway was dominant during the fermentation when pH was not controlled. By controlling the pH at 7 this pathway can be shifted to a mixed acid pathway, which is favorable to the production of hydrogen.
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KEYWORD
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Enterobacter sp. SNU-1453, pH, metabolic flux shift
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